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For the C. elegans elongation factor 1α gene (Mitrovich and Anderson, 2000)eft-3 promoter1625 .. 2224 = 600 bppromoter for the C. elegans elongation factor 1α gene (Mitrovich and Anderson, 2000)ori834 .. 1422 = 589 bphigh-copy-number ColE1/pMB1/pBR322/pUC origin of replicationori834 .. 1422 = 589 bphigh-copy-number ColE1/pMB1/pBR322/pUC origin of replicationCeU6 promoter6967 .. 7316 = 350 bppromoter for the C. elegans R07E5.16 U6 snRNA gene (Dickinson et al., 2013)CeU6 promoter6967 .. 7316 = 350 bppromoter for the C. elegans R07E5.16 U6 snRNA gene (Dickinson et al., 2013)tbb-2 terminator6575 .. 6898 = 324 bptranscription terminator for the C. elegans tubulin beta-2 genetbb-2 terminator6575 .. 6898 = 324 bptranscription terminator for the C. elegans tubulin beta-2 geneAmpR promoter7811 .. 7915 = 105 bpAmpR promoter7811 .. 7915 = 105 bpgRNA scaffold7318 .. 7393 = 76 bpguide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 systemgRNA scaffold7318 .. 7393 = 76 bpguide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 systemattB41604 .. 1624 = 21 bpcore recombination site for the Gateway® BP reactionattB41604 .. 1624 = 21 bpcore recombination site for the Gateway® BP reactionattB36902 .. 6922 = 21 bpcore recombination site for the Gateway® BP reactionattB36902 .. 6922 = 21 bpcore recombination site for the Gateway® BP reactionORF: 7916 .. 663 = 861 bpORF: 286 amino acids = 31.6 kDaORF: 2241 .. 5516 = 3276 bpORF: 1091 amino acids = 126.9 kDaORF: 5658 .. 6563 = 906 bpORF: 301 amino acids = 34.3 kDaORF: 267 .. 533 = 267 bpORF: 88 amino acids = 9.2 kDaClick here to try SnapGeneDownload pDD162 (Peft-3_Cas9 + Empty sgRNA).dna fileSnapGeneSnapGene is the easiest way to plan, visualize and document your everyday molecular biology proceduresFast accurate construct design for all major molecular cloning techniquesValidate sequenced constructs using powerful alignment toolsCustomize plasmid maps with flexible annotation and visualization controlsAutomatically generate a rich graphical history of every edit and procedureSnapGene ViewerSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.Gain unparalleled visibility of your plasmids, DNA and protein sequencesAnnotate features on your plasmids using the curated feature databaseStore, search, and share your sequences, files and mapsIndividual Sequences & MapsPlasmid SetsThe maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.Discover the most user-friendly molecular biology experience.

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